Table 1.
Monocyte/macrophage-based miRNAs as biomarker candidates for TB.
| Cells | Differentially Expressed miRNAs | Analysis Platform | Reference |
|---|---|---|---|
| Human | |||
| MDMs infected with MTB or BCG | miR-155, miR-146a, miR-145, miR-222, miR-27a, and miR-27b | Taqman low-density array | (110) |
| MDMs from TB patients, LTB, and Healthy individuals | TB vs HC: | Taqman microarray quantitative PCR | (71) |
| Upregulated (hsa-miR-16, hsa-miR-137, hsa-miR-140-3p, hsa- miR-193a-3p, hsa-miR-501-5p, and hsa-miR-598) | |||
| Downregulated (hsa-miR-95) | |||
| LTB vs TB: Upregulated (hsa-miR-101 and hsa-miR-150) | |||
| Unique expression in LTB (miR-146b-3p and hsa-miR-296-5p) | |||
| MDM infected with TB LM | miR-125-b | qPCR | (50) |
| MDM infected with M. smeg LM | miR-155 | ||
| MDM infected with MTB H37Rv | Upregulated (miR-155, miR-21, miR-146a, miR-29a, miR-26a, let-7b, miR-34, miR-132 & miR-138) | Nanostring nCounter miRNA assay | (65) |
| Downregulated (miR-660, miR-144, miR-301b, miR-128, miR-423-3p, miR-410, miR-27a, miR-93, miR-107, miR-345, miR-221, miR-25, miR-23b, miR-361-5p, miR-130b & miR-340) | |||
| MDM infected with MTB | Upregulated (miR-132, miR-146-5p, miR-30e, let-7i, miR-490-3p, miR-29c, miR-26a, miR-21, let-7b & miR-29a) | Nanostring nCounter miRNA assay | (57) |
| Downregulated (miR-25, miR-23b, miR-331-3p, miR-423-3p, miR-548f, miR-340, miR-24, miR-107, miR-93, miR-324-5p, miR-188-5p, miR-130b, miR-410, miR-361-5p, miR-197, mir-27a, miR-128, miR-345, miR-379, miR-133a & miR-221. | |||
| Primary monocytes and MDMs from active TB patients and controls | Upregulated-miR-582-5p | qPCR | (75) |
| Primary macrophages from TB patients vs controls | Upregulated miR-223 | qPCR | (62) |
| Macrophages from TB patients and controls | Downregulated miR-365 | qPCR | (67) |
| MDM infected with MTB | Upregulated miR-106b-5p | qPCR | (70) |
| Mouse | |||
| BMDMs infected with MTB | 6 upregulated (miR-21, miR-21*, miR-146a, miR-146 b, miR210, and miR-155), 1 downregulated (miR-223) | Microarray and qPCR | (111) |
| BMDMs infected with Mtb | 4 upregulated (miR-24, miR-142, miR-155, and miR-212) and 3 downregulated (miR-19a, miR-202, and miR-376a) | Gene expression microarray | (112) |
| BMDMs infected with BCG | miR-21 | Taqman quantitative real-time PCR | (63) |
| BMDMs infected with MTB | Upregulated miR-27b | qPCR | (79) |
| BMDMs infected with MTB | 3 upregulated (miR-155, miR-146a & miR-21) | Taqman low-density arrays | (1) |
| Mouse peritoneal macrophages & BMDMs | Upregulated miR-146a | qPCR | (60) |
| Cell Line | |||
| U937 macrophages | 149 DE (miR-424–5p, miR-493-5p, miR-27 b-3p, miR-296-5p, miR-377–5p, miR-3680–5p) | Microarray | (113) |
| THP-1 cells infected with Beijing/W or non-Beijing/W strains | 13 downregulated (let-7e, let-7f, miR-10a, miR-21, miR-26a, miR-99a, miR-140–3p, miR-150, miR-181a, miR-320, miR-339–5p, miR-425, and miR-582-5p) | Taqman microarray quantitative PCR | (71) |
| THP-1 cells infected with virulent or avirulent Mtb strains | 9 DE (miR-30a, miR-30e, miR-155, miR-1275, miR-3665, miR-3178, miR-4484, miR-4668-5p, and miR- 4497) | Microarray | (114) |
| THP-1 cells infected with MTB HN878 | 12 upregulated (miR-33b*, miR-146a, miR-155, miR-132, miR-146b-5p, miR-720, miR-30e, miR-661, miR-140-3p, miR-3651, miR-328, and miR-378 | (115) | |
| THP-1 cells and U937 cells | Upregulated miR-32-5p | qPCR | (66) |
| THP-1 cells | Upregulated miR-30a | qPCR | (55) |
| RAW264.7 cells and infected with MTB | 3 upregulated (miR-155, miR-146a, and miR-21) | Taqman low-density arrays | (1) |
| RAW264.7 cells infected with MTB | Upregulated miR-27b | qPCR | (79) |
| RAW264.7 cells infected with MTB | Downregulated let-7f | SYBR Green-based miRNA profiling array | (58) |
| RAW264.7 cells | Downregulated miR-20b-5p | Semi quantitative PCR | (72) |