Table 1.
Primer sequences, amplicon characteristics, efficiency of different gene for RT-qPCR analysis.
| Gene symbol | Accession number | Primer sequences (5′−3′) | Amplicon size (bp) | TM (°C) | Efficiency (%) |
|---|---|---|---|---|---|
| PC | NM 204346.1 | F: AAGACGCTGCACATCAAAGC | 113 | 60 | 104.13 |
| R: TGGGTGTCTCTCACGAGGAT | |||||
| PEPCK | NM 205471.1 | F: GGCAATCAATCCAGAAAACG | 139 | 56 | 109.33 |
| R: CCAATAGACACCCCCATCAC | |||||
| FBP | NM 001278048.1 | F: GAATAGCAACAACAGGGCATC | 81 | 58 | 106.01 |
| R: ATCCCAAGACAACAGGCACT | |||||
| GYS1 | XM 025145463.1 | F: CACGCACCAACAACTTCAAC | 122 | 58 | 108.23 |
| R: CACCAGCAGCGACTCATAGA | |||||
| PYG | NM 204392.1 | F: CTTTGGGATGAGGGTGGAG | 105 | 58 | 109.17 |
| R: ATCTGGTCAACTGCCTGCTT | |||||
| IL 1 | XM 015297469.1 | F:GCATCAAGGGCTACAAGCTC | 131 | 59 | 100.19 |
| R:CAGGCGGTAGAAGATGAAGC | |||||
| IL 2 | XM 015276098.2 | F: ACCGGAAGTGAATGCAAGAT | 212 | 58 | 106.53 |
| R: AGTGGTCCCAGAATGGACAG | |||||
| IL 4 | NM 001007079.1 | F: GCTCTCAGTGCCGCTGATG | 61 | 60 | 105.63 |
| R: GGAAACCTCTCCCTGGATGTC | |||||
| IL 6 | XM 015281283.2 | F: CTCCTCGCCAATCTGAAGTC | 100 | 59 | 105.64 |
| R: CCCTCACGGTCTTCTCCATA | |||||
| IL 10 | XM 025143715.1 | F: CTGAAGGCGACGATGC | 406 | 55 | 101.43 |
| R:TTCCTCCTCCTCATCAGC | |||||
| TNF.α | XM 015294125.2 | F:AGGCCAGATGGGAAGGGAATGAA | 219 | 63 | 104.14 |
| R:GAAGAGGCCACCACACGACAG | |||||
| GAPDH | NM 204305.1 | F:CACCCTCAAGATTGTCAGC | 98 | 60 | 109.32 |
| R:TAAGTCCCTCCACGATGC |
Abbreviations: FBP, fructose-1,6-bisphosphatase; GYS1, liver glycogen synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1, Interleukin 1; IL2, Interleukin 2; IL4, Interleukin 4; IL6, Interleukin 6; IL10, Interleukin 10; PC, pyruvate carboxylase; PEPCK, phosphoenolpyruvate carboxykinase; PYG, glycogen phosphorylase, TNF.α, tumor necrosis factor α.