Supplementary Figure 7.

Increased DLST expression and effect of glutamine deprivation on upregulated TCA cycle genes. (A) Representative dot plot graph of 5-fold change of all the genes in the glucose metabolism PCR array (B) Bar graph represents the fold change in both downregulated and upregulated genes from glucose metabolism array. (B) Increased DLST expression validated individually by immunoblotting in CD11b⁺Gr1⁺ myeloid cells from GMCSF/IL6 and GMCSF/IL6+ ID8 CM and (D) Real-time PCR was performed on key upregulated genes Succinyl Co A ligase (SUCLG2), Succinate dehydrogenases (SDHA-SDHD) (from glucose metabolism array) under conditions of low and regular glutamine conditions (n = 3). ∗p < 0.05 low glutamine compared to regular glutamine as assessed by unpaired T-test.