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. 2021 Jun 26;22(13):6889. doi: 10.3390/ijms22136889

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Effect of CD14+ monocyte and CD3+CD4+ lymphocyte subpopulations on the formation of cytotoxic activity of Hsp70-activated PBMCs. These subpopulations were removed by magnetic separation, PBMCs were treated with Hsp70 for six days, and cytotoxicity of activated lymphocytes was measured after 24 h incubation with target cells. PBMCs incubated with control protein (BSA) were used as negative control. The data are presented as the mean ± SD of three independent experiments. (B) The levels of IL-2, TNFα, and IFNγ mRNAs measured by qPCR in monocytes incubated with Hsp70 for 3 h. (C) Dynamics of IL-2 secretion by PBMCs incubated with Hsp70 or LP17 + Hsp70 for six days.