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. 2021 Jul 2;22(13):7166. doi: 10.3390/ijms22137166

Figure 4.

Figure 4

Human vascular cell adhesion molecule-1 (hVCAM1) expression was down-regulated by hBAFF-siRNA. (A–C) MH7A cells were transfected with control or hBAFF-siRNA to knockdown hBAFF expression by using Lipofectamine®. Cells were treated with 20 ng/mL TNF-α. RNA was purified with Nucleozol®. hVCAM1 and hBAFF expression was detected by RT-PCR (A). Cells were transfected with pEZx-PG02-hVCAM1 gaussia luciferase (Gluc) plasmid DNA by using PEI, and treated with 20 ng/mL TNF-α. Gluc activity was measured by using luminometer (B). Cells were treated with 20 ng/mL TNF-α for 6 h. Then, WiL2-NS cells were added and co-incubated to adhere for 30 min. Unbound WiL2-NS B cells were washed out and MTT assay was used to analyze bound B cells (C). Each experiment was performed at least four times. Data in a bar graph represent the means ± SD. ** p < 0.01; significantly different from control-siRNA-treated and TNF-α-untreated control group. & p < 0.01, && p < 0.01; significantly different from control-siRNA-treated and TNF-α-treated control group (A,C).