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. 2021 Jun 30;22(13):7092. doi: 10.3390/ijms22137092

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Osteogenic markers in human adipose stem cells (hASCs) cultured on biomorphic HA (B-HA), sintered HA (S-HA) biomaterials. (A) Alizarin Red staining is shown in this panel, in experimental conditions tested (10× magnification upper figures, 4x magnification lower figures). (B) The matrix mineralization was evaluated by Alizarin red staining, whereas its quantification was carried out spectrophotometrically. * p < 0.05; ** p < 0.01 (ANOVA test). Matrix mineralization data was reported as optical density. (C) The osteocalcin (OCN) protein levels were quantified by ELISAs. OCN protein was reported as ng of Osteocalcin/μg of total protein. * p < 0.05, ** p < 0.01.