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. 2021 Jun 30;22(13):7092. doi: 10.3390/ijms22137092

Figure 4.

Figure 4

RT2 PCR Array analyses of osteogenic genes. Gene expression was evaluated in human adipose stem cells (hASCs) grown on (A) biomorphic HA (B-HA) and (B) sintered HA (S-HA), compared to tissue culture polystyrene (TCPS) at day 14. (A) In hASC cultures, mRNAs from 17 genes in the osteogenic pathway, i.e., ALPL, BGLAP, CHRD, COL10A1, CSF3, EGF, FGFR2, GLI1, ITGA3, MMP10, MMP8, MMP9, SMAD3, SOX9, SP7, TGFB3 and TNFSF11 were up-regulated (red). (B) In hASC cultures, mRNAs of 10 genes in the osteogenic pathway, i.e., BMP2, COL2A1, CSF2, EGF, GLI1, MMP10, MMP8, SP7, SPP1, TNFSF11 were up-regulated (red), while 16 tested genes resulted down-regulated (green): BGLAP, BGN, BMP6, COL1A1, COL1A2, COL3A1, COL5A1, FGF1, FN1, IGF1, ITGA2, ITGA3, ITGB1, MMP2, TGFB3 and VCAM1. (C) Fold change (FC) of common up-regulated gene EGF, GLI1, MMP10, MMP8, SP7, TNFSF11. (D) Genes modulated by B-HA and S-HA in hASC cultures at day 14 are represented by Venn diagram. The common genes up-regulated from both biomaterials are indicated.