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. 2021 Jul 4;22(13):7204. doi: 10.3390/ijms22137204

Table 2.

Antibodies used for immunohistochemical analysis.

Primary Antibody (Clone No.) Supplier (Location)
MiTF (Clone D5), MoMoAb, 1:100 Dako, Agilent Technologies, Inc. (Santa Clara, CA, USA)
HMB45 (Clone HMB-45), MoMoAb, 1:100
MiTF (Clone D5), MoMoAb, 1:100
MELAN A (A103), MoMoAb, 1:100
CD68 (M0814), MoMoAb, 1:100
CD45 (SAB4502541) RaMoAb, 1:200 Sigma-Aldrich, Prague, Czech Republic
CD8 (Clone SP239), RaMoAb, 1:100
Secondary Antibody (Clone No.) Supplier (Location)
N-Histofine Simple Stain MAX PO (414152F) EXBIO Prague s.r.o. (Prague, Czech Republic)
Chromogen Supplier (Location)
DAB (3,3′-Diaminobenzidine) Dako, Agilent Technologies, Inc. (Santa Clara, CA, USA)
MoMoAb, Mouse Monoclonal Antibody; RaMoAb, Rabbit Monoclonal Antibody; RaPoAb, Rabbit Polyclonal Antibody