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. 2021 Jun 24;22(13):6777. doi: 10.3390/ijms22136777

Figure 2.

Figure 2

Expression of select fibrotic factors via immunohistochemistry. (A–C). TGF-β1 (A), collagen type 1 (B) and α-smooth muscle actin (C) expression at 1, 2, and 4 weeks. It can be visually determined that the fibrotic effect is reduced with treatment of human umbilical cord blood-derived mesenchymal stem cells (hMSCs) as opposed to CCl4 induced cirrhosis and saline. Mice livers injected with saline (left), olive oil and hMSC injection (left center), CCl4 induced cirrhosis with saline injection (right center) and CCl4 induced cirrhosis with hMSC injection (right) on each of the three panels. The MSC injection group shows a 4-fold decrease in αSMA and TGF-β and a 3-fold decrease in collagen 1 at 4 weeks with n = 5 mice. The study demonstrates MSC homing into the cirrhotic livers more so compared to healthy livers, further signifying the efficacy of the cellular therapy. Original magnification of 200×. Values are presented as mean ± SEM, for at least five separate experiments. Data are presented as fold change with respect to saline/CCl4 group, * p < 0.05, ** p < 0.01. Reprinted with permissions from Jung et al. [79].