Figure 5.

Datelliptium downregulates cyclin D1 protein expression levels in TT- and MZ-CRC-1 cells. (A) Cells were treated with datelliptium for 48 h at several concentrations, and the levels of phosphorylated and total mTOR protein were measured by western blotting. (C) TT- and MZ-CRC-1 cells were treated for 48 h with indicated concentrations of datelliptium and harvested to get protein lysates. The cellular lysates prepared from TT- and MZ-CRC-1 cells were analyzed by western blot using specific antibodies against cyclin D1, Rb, or pRb protein. Actin served as a control for equal loading. (B,D) Densitometry analysis of the western blotting of (A,C), respectively. pmTOR, mTOR, cyclin D, and pRb/Rb were normalized to their basal expression (n = 3, **** p < 0.0001, *** p < 0.001, and ** p ≤ 0.01).