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. 2021 Jun 28;13(13):3233. doi: 10.3390/cancers13133233

Figure 5.

Figure 5

Effects of miR-222 depletion on the expression of selected miR-222 target genes. (A) qRT-PCR expression analysis of bioinformatically identified miR-222 target genes in PANC-1 or MIA PaCa-2 cells 24 h PI with 10 IFU/cell of AdNuPAR-E-Scramble or AdNuPAR-E-miR222-S. (B) qRT-PCR expression analysis of bioinformatically identified miR-222 target genes in Ctrl and CRISPR miR-222 PANC-1 KO and MIA PaCa-2 KO cells. Data are shown as mean ± SEM for at least five independent biological replicates. Significance was assessed using a two-tailed Mann-Whitney test. * p < 0.05, ** p < 0.01.