Skip to main content
. 2021 Jun 25;12:689902. doi: 10.3389/fgene.2021.689902

FIGURE 3.

FIGURE 3

Spermatogenesis in Ubl4b knockout mice. (A) Western blot analysis of UBL4B expression in testes from 10-week-old WT and Ubl4b–/– mice. β-Actin was used as the loading control. (B) Representative image of testes from 10-week-old WT and Ubl4b–/– mice. (C) Ratios of testes to body weight were determined from 10-week-old WT and Ubl4b–/– mice. (D) H&E staining of testes from 10-week-old WT (a,b) and Ubl4b–/– (c, d) mice. Scale bars, 50 μm. (E) H&E staining of caput and cauda epididymides from 10-week-old WT (a, b) and Ubl4b–/– (c,d) mice. Scale bars, 50 μm. (F) Average sperm count in unilateral epididymis from 10-week-old WT and Ubl4b–/– mice. (G) H&E staining of sperm in cauda epididymides from 10-week-old WT and Ubl4b–/– mice. Scale bars, 20 μm. (H) Percentages of sperm with normal morphology were shown. (I,J) Percentages of motile sperm (I) and progressively motile sperm (J) from 10-week-old WT and Ubl4b–/– mice. n, the number of animals. The data shown were represented as the mean ± SEM. Student’s t-test was performed between WT and Ubl4b–/– mice. NS, no significant difference.