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Fertility assay.
| Genotype | Mating period (months) | No. of fertile males/No. of mice tested | Average pups/litter | Offspring sex ratio (% males) |
| WT | 3 | 3/3 | 7.60 ± 0.23 | 0.52 ± 0.09 |
| Ubl4a–/Y; Ubl4b–/– | 3 | 3/3 | 7.91 ± 0.56NS | 0.56 ± 0.08NS |
Each male was engaged with two WT females. Student’s t-test was used for comparison of average pups/litter and offspring sex ratios (% males) between WT and Ubl4a–/Y; Ubl4b–/– mouse groups. NS, no significant difference. Data were presented as mean ± SEM.
