FIGURE 1.

Development of tolerance to the anti-allodynic effects of 6 mg/kg of ∆⁹-THC in male and female wild-type and S426A/S430A mice. Tolerance to the anti-allodynic effects of 6 mg/kg ∆⁹-THC was determined in both male (A) and female (B) S426A/S430A (KI; unfilled) and wild-type (WT; filled) mice. Mice were assessed via the von Frey assay for the amount of force (in grams) required to elicit a paw withdrawal response 60 min following treatment with 6 mg/kg ∆⁹-THC. BL represents the pre-cisplatin baseline and CISP the post-cisplatin baseline. Error bars represent the mean ± SEM. Each mouse was tested in triplicate and those values averaged to determine a single value for each mouse each day of testing. Data were analyzed using separate two-way ANOVAs with Bonferroni post-hoc tests. Sample sizes for each group (shown in parentheses) include 26 male (13 WT; 13 KI) and 27 female (13 WT; 14 KI) mice.