FIGURE 3.

Development of tolerance to the anti-allodynic effects of 10 mg/kg of ∆⁹-THC in female wild-type and S426A/S430A mice. Results from a dose-response (insert) revealed that 10 mg/kg of ∆⁹-THC fully reversed allodynia in wild-type females. Tolerance to the anti-allodynic effects of 10 mg/kg ∆⁹-THC was determined in female S426A/S430A (KI; unfilled) and wild-type (WT; filled) mice. Mice were assessed using the von Frey assay for the amount of force (in grams) required to elicit a paw withdrawal response 60 min following treatment with 6 mg/kg ∆⁹-THC. BL represents the pre-cisplatin baseline and CISP the post-cisplatin baseline. Error bars represent the mean ± SEM. Each mouse was tested in triplicate and those values averaged to determine a single value for each mouse each day of testing. Data were analyzed using separate two-way ANOVAs with Bonferroni post-hoc tests. Sample sizes for each group (shown in parentheses) include 13 female WT mice for the dose response and 13 female WT and 14 female KI mice for daily tolerance.