FIGURE 4.

Development of tolerance to the anti-allodynic effects of 10 mg/kg of ∆⁹-THC in male and female wild-type mice. Tolerance to the anti-allodynic effects of vehicle (unfilled) and 10 mg/kg (filled) ∆⁹-THC was determined in both male (squares) and female (circles) wild-type (WT) mice using the von Frey. Mice were first assessed for the amount of force (in grams) required to elicit a paw withdrawal response 60 min following treatment with vehicle and 60 min later following treatment with 10 mg/kg ∆⁹-THC. BL represents the pre-cisplatin baseline and CISP the post-cisplatin baseline. Error bars represent the mean ± SEM. Each mouse was tested in triplicate and those values averaged to determine a single value for each mouse for each dose tested on each day of testing. Data were analyzed using separate two-way ANOVAs with Bonferroni post-hoc tests. Sample sizes for each group (shown in parentheses) include 15 male and 16 female WT mice.