FIGURE 5.

Development of tolerance to equally efficacious doses of ∆⁹-THC in male and female wild-type mice. Tolerance to the anti-allodynic effects of vehicle (unfilled) and equally efficacious doses of ∆⁹-THC (filled) was determined in both male (squares) and female (circles) wild-type (WT) mice using the von Frey. Mice were first assessed for the amount of force (in grams) required to elicit a paw withdrawal response 60 min following treatment with vehicle and 60 min later following treatment with 10 mg/kg (female) or 6 mg/kg (male) of ∆⁹-THC. BL represents the pre-cisplatin baseline and CISP the post-cisplatin baseline. Error bars represent the mean ± SEM. Each mouse was tested in triplicate and those values averaged to determine a single value for each mouse for each dose on each day testing. Data were analyzed using separate two-way ANOVAs with Bonferroni post-hoc tests. (**p > 0.01; ***p < 0.001; compared to females on the same day). Sample sizes for each group (shown in parentheses) include 15 male and 15 female WT mice.