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. 2021 Jun 25;9:665886. doi: 10.3389/fcell.2021.665886

FIGURE 4.

FIGURE 4

Dentin matrix protein staining and osteo-odontogenic gene expression in CopGFP control and VEGFR-2 shRNA DPSCs after a culture in osteo-odontogenic BMP-2 media. Immunocytochemistry showed certain positive cells of anti-DMP-1 (A) and anti-DSP (B) staining (in brown) in differentiated CopGFP control DPSCs but slightly stained cells in VEGFR-2 shRNA DPSCs (D,E). The rabbit IgG control (Rb IgG Ctrl) stained in CopGFP control (C) and VEGFR-2 shRNA DPSCs (F) were completely negative to confirm the specificity of antibodies. Scale bars indicate 100 μm. The bar graph reveals that real-time PCR analysis showed higher expression of Dmp-1, Dspp, and Bsp in differentiated CopGFP control DPSCs, compared to that in differentiated VEGFR-2 shRNA treated DPSCs (G). Differences in gene expression is demonstrated as the fold change of that in differentiated VEGFR-2 shRNA DPSC group. GAPDH was used as the internal control. * is used for significantly statistical difference at p-value < 0.05. The results represent the means ± SD of three independent experiments.