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. 2021 Jun 25;9:665886. doi: 10.3389/fcell.2021.665886

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Copyright © 2021 Janebodin, Chavanachat, Hays and Reyes Gil.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alkaline phosphatase and Alizarin Red S staining in CopGFP control and VEGFR-2 shRNA DPSCs after a culture in BGP + Vit. C media and VEGF media. After induction in BGP plus vitamin C, some differentiated CopGFP DPSCs (A,C,E) but not VEGFR-2 shRNA DPSCs (B,D,F) demonstrated positive staining of alkaline phosphatase. Alizarin Red S staining showed some positive mineralized nodules in CopGFP DPSCs but not VEGFR-2 shRNA DPSCs (M). For VEGF induction, a similar results of alkaline phosphatase staining were observed in both CopGFP control (G,I,K) and VEGFR-2 shRNA DPSCs (H,J,L), compared to the BGP plus vitamin C. Interestingly, no mineralized nodule was found in both cell groups after VEGF induction (N). Scale bars indicate 100 μm.