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Positive ion-mode ESSI mass spectra of cytochrome c: mass spectrum of the intact protein dissolved in 4% formic acid (A), in-spray digestion of cytochrome c according to experiment 1A (B), mass spectrum of the reacidified, ammonia-quenched reaction mixture from the control experiment 1B (C), and mass spectrum of cytochrome c after 3 h of pepsin digestion in the bulk phase (D). For details, see the Experimental Section and the Supporting Information.
