Figure 3.

Increased fat mass and decreased lean mass for OGTHet^Pl female mice but no difference in energy balance on HFD. (A) Daily food intake of HFD averaged over three days for female mice (n = 4, 8). (B) Lean and (C) fat mass of females (n = 4, 7) after 16 weeks HFD as assessed by EchoMRI. (D) Activity evaluated by beam breaks, (E) Volume of oxygen (VO₂) consumption, and (F) volume of carbon dioxide (VCO₂) generation over 72 h (n = 4, 8) with 12 h light and dark cycles. Dark cycle designated by the shaded region. (G) Respiratory exchange ratio (RER) over 72 h (n = 4, 8) with 12 h light and dark cycles. Dark cycle designated by the shaded region. (H) Averaged RER for light and (I) dark cycles (n = 4, 8). (J) Energy expenditure (EE) over 72 h (n = 4, 8) with 12 h light and dark cycles. Dark cycle designated by the shaded region. (K) Averaged EE for light and (L) dark cycles (n = 4, 8). (A–C,H,I,K,L) was presented as mean ± SEM, unpaired t-test. (D–F,G,J) were presented as mean, two-way ANOVA. p values, ** p < 0.01.