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. 2021 Jun 25;11:672684. doi: 10.3389/fonc.2021.672684

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Copyright © 2021 Zhang, Li, Zhao, Yang, Sang, Yan, Wang and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M2-TAM-Exo overexpressing miR-183-5p promoted the malignant phenotype in CC cells. Cisplatin (5 μm) was used for inducing apoptosis of LoVo and SW480 cells. M2-TAM overexpressing miR-183-5p were co-cultured with LoVo and SW480 cells, respectively. (A) The miR-183-5p level in M2-TAM was monitored by qRT-PCR. (B) The miR-183-5p profile in LoVo and SW480 cells was monitored by qRT-PCR. (C, D) CCK-8 assay and colony formation assay were implemented to test CC cell proliferation. (E) The ability of cell colony formation was tested. (F) Wound healing test detected cell migration. (G) Cell invasion was measured by Transwell assay. (H) Cell apoptosis was verified by FCM. *P <0.05, **P < 0.01, ***P < 0.001 (vs. The M2-TAM ^miR-NC group). N=3.