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miR-183-5p targeted THEM4. (A) Bioinformatics predicted the potential binding sites between miR-183-5p and THEM4. (B) The THEM4 level in LoVo and SW480 cells was monitored by qRT-PCR. (C) The targeting association between miR-183-5p and THEM4 was determined by the dual-luciferase reporter assay in SW480 and LoVo. (D) The expression of THEM4, Akt and NF-κB was compared by WB. ns, P >0.05, **P < 0.01, ***P < 0.001 (vs. The M2-TAM miR-NC group). N=3.
