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. 2021 Jun 25;9:608490. doi: 10.3389/fcell.2021.608490

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Copyright © 2021 Garcillán, Fuentes, Marin, Megino, Chacon-Arguedas, Mazariegos, Jiménez-Reinoso, Muñoz-Ruiz, Laborda, Cárdenas, Fernández-Malavé, Toribio and Regueiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Generation of CD3G or CD3D KD T-cell lines. Jurkat E6-1 cells were transduced with five different shRNA for CD3γ or CD3δ and one non-target shRNA (shNT). Cells were lysed in NP-40 buffer after 7 days in culture and 40 μg of total protein was loaded. Membranes were incubated with antibodies against alpha tubulin (Sigma), CD3γ (Santa Cruz Biotechnology), or CD3δ (Santa Cruz Biotechnology). Numbers below each panel indicate the percentage of each chain expression relative to shNT normalized to the loading control (tubulin).