FIGURE 2.

CD3 protein expression in CD3G or CD3D KD T-cell lines. Jurkat E6-1 cells were transfected with shCD3γ, shCD3δ, and shNT. Cells were lysed in NP-40 buffer after several days in culture with puromycin and 40 μg of total protein was loaded. Membranes were incubated with antibodies against tubulin (Sigma) and (A) CD3γ (Abcam), (B) CD3δ (Santa Cruz Biotechnology), or (C) CD3ε (Abcam). Band analysis and numbers as in Figure 1. Left: a representative experiment. Right: quantification of at least 10 different experiments; data are mean values + SD. Tg, totally glycosylated; pg, partially glycosylated. Note: all glycosylated forms of CD3γ were quantified together.