FIGURE 5.

Co-IP of ζζ/CD247₂ and other TCR chains in CD3G or CD3D KD T-cell lines. (A) Cells were lysed in Brij96V buffer, 3 mg of total protein was immunoprecipitated with anti-TCRβ mAb (JOVI-1), and the immunoprecipitates and whole lysates were resolved in 15% SDS-PAGE gels and immunoblotted with antibodies against ζζ/CD247₂ (448) or alpha tubulin (Sigma). (B) Immunoprecipitation was done with anti-ζ and immunoblot with mAb against CD3ε (Abcam) or α-tubulin (Sigma). Band analysis and numbers as in Figure 1. The tubulin loading control is shared in (A,B) for illustrative purposes.