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. 2021 Jun 25;9:608490. doi: 10.3389/fcell.2021.608490

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Copyright © 2021 Garcillán, Fuentes, Marin, Megino, Chacon-Arguedas, Mazariegos, Jiménez-Reinoso, Muñoz-Ruiz, Laborda, Cárdenas, Fernández-Malavé, Toribio and Regueiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD3 KD and surface TCR expression correlate in T cells other than Jurkat E6-1. (A) Comparative intracellular (i) CD3γ or CD3δ expression and extracellular (e) CD3ε expression in the indicated T cells transduced with shCD3γ, shCD3δ, or shNT. Vertical lines indicate the upper limit staining of the isotype control. Numbers indicate % expression vs. control (shNT). (B) Correlation between CD3γ or CD3δ chain expression and surface TCR, expressed as % of expression vs. shNT. Data in (A) (red symbols) in comparison with Jurkat E6-1 data (black dots) and comparative linear regression. The slopes are not significantly different (P = 0.9046).