FIGURE 9.

Fetal thymus organ cultures (FTOC) of shCD3δ-3 vs. shNT-transduced human T-cell progenitors. Top and middle: mouse embryo thymic lobes were seeded with shCD3δ-3 or shNT-transduced CD34^hi early T-cell progenitors from human thymus and cultured for up to 5 weeks. T-cell generation was analyzed on electronically gated GFP⁺ transduced cells recovered from different lobes pooled at the indicated time points in two independent experiments. Bottom: representative (FTOC 2) flow cytometry analysis of CD3 vs. αβTCR expression on electronically gated GFP⁺ cell progenies at the indicated days. Numbers in biparametric histograms indicate percentages of CD3⁺ αβTCR⁺ (αβ T cells), CD3^–/low αβTCR^– (including pre-TCR⁺ pre-T cells) and CD3⁺ αβTCR^– (γδ T cells). Percentages of γδ T cells were independently confirmed by CD3 vs. γδTCR expression analysis. Numbers in monoparametric histograms indicate MFI values for each indicated subset.