Table 1.

Immune response desirability weighting.

Biological sample	Assay type	Assay target	Weight (1-5, 5 being most important)	Function	Rationale
Spleen	Cytokine bead array	IFN-γ	5	Maximize	IFNγ correlates with protection in humans and mice (6, 12)
Stool	ELISA	IgA	5	Maximize	Fecal IgA correlates with protection in humans (5)
Spleen	Cytokine bead array	IL-17A	4	Maximize	Depletion of IL-17A increases susceptibility in mice (7)
Bone marrow	ELISpot	IgA	3	Maximize	Plasma cells are necessary for durable IgA mediated immunity (13)
Bone marrow	ELISpot	IgG	2	Maximize	Plasma cells are necessary for durable IgG mediated immunity (13)
Blood	ELISA	IgG	2	Maximize	Serum IgG antibody titers are indicative of systemic immunogenicity (14)
Blood	ELISA	IgG2a/IgG1 ratio	2	Maximize	IgG2a/IgG1 ratio correlates with Th1 immunity (15)