Table 4.

Desirability index scores of each experimental group in the second immunogenicity experiment based on immunogenicity and adjuvant physicochemical stability readouts.

Group #	Description*	IFN-γ	Fecal IgA	IL-17A	Bone Marrow ASC IgA	Bone Marrow ASC IgG	serum IgGT	serum IgG2a/IgG1 ratio	Immuno-genicity Score (female)	Immuno-genicity Score (male)	Overall Immuno-genicity Score	Overall Adjuvant Stability Score**	Combined Overall Score
7	PO + antioxidant	0.984	0.981	0.861	0.919	0.914	0.822	0.990	0.896	0.920	0.932	0.886	0.909
5	PO + tonicity agent	0.971	0.990	0.868	0.839	0.752	0.746	0.590	0.854	0.833	0.859	0.683	0.766
15	DPPC, Mid ratio	0.932	0.874	0.871	0.924	0.937	0.912	0.493	0.903	0.778	0.857	0.669	0.757
3	PO	0.912	0.936	0.621	0.845	0.868	0.740	0.736	0.834	0.757	0.815	0.687	0.749
19	DSPC, Mid ratio	0.963	0.715	0.873	0.861	0.919	0.823	0.745	0.941	0.703	0.840	0.598	0.709
12	DMPC, Mid ratio	0.978	0.762	0.844	0.694	0.691	0.990	0.433	0.765	0.771	0.781	0.628	0.700
17	DOPC, High ratio	0.975	0.908	0.886	0.721	0.795	0.904	0.393	0.720	0.874	0.818	0.591	0.696
11	DMPC, Low ratio	0.952	0.858	0.681	0.782	0.867	0.858	0.614	0.844	0.749	0.810	0.562	0.675
16	DSPC, High ratio	0.987	0.842	0.990	0.832	0.850	0.878	0.497	0.817	0.877	0.859	0.526	0.672
8	DPPC, Low ratio	0.990	0.863	0.818	0.653	0.689	0.829	0.863	0.687	0.898	0.830	0.450	0.611
9	DOPC, Mid ratio	0.973	0.658	0.695	0.649	0.786	0.737	0.633	0.788	0.666	0.738	0.504	0.610
14	DSPC, Low ratio	0.971	0.971	0.935	0.990	0.990	0.936	0.376	0.931	0.826	0.889	0.369	0.573
10	DOPC, Low ratio	0.974	0.796	0.755	0.831	0.855	0.801	0.523	0.792	0.775	0.804	0.241	0.440
2	POC	0.890	0.960	0.558	0.815	0.801	0.651	0.575	0.780	0.725	0.766	0.231	0.420
13	DPPC, High ratio	0.966	0.962	0.921	0.981	0.963	0.946	0.128	0.891	0.697	0.803	0.195	0.396
18	DMPC, High ratio	0.967	0.719	0.694	0.870	0.866	0.814	0.559	0.735	0.804	0.785	0.137	0.328
20	PSO (less LecA)	0.862	0.010	0.281	0.135	0.247	0.104	0.027	0.077	0.161	0.116	0.669	0.279
4	PSO (less 3M052)	0.809	0.655	0.314	0.495	0.462	0.452	0.010	0.570	0.305	0.379	0.108	0.203
6	PO + viscosity agent	0.947	0.814	0.699	0.663	0.648	0.777	0.674	0.722	0.781	0.766	N/A	N/A
1	LecA alone	0.010	0.041	0.010	0.010	0.010	0.010	0.195	0.013	0.023	0.017	N/A	N/A

LecA dose was 10 µg for all groups except for the POC formulation (5 µg) and the PSO-less LecA (0.5 µg). *PO, Predicted Optimal formulation from dose optimization DOE; PSO, Predicted Sub-Optimal formulations from dose optimization DOE; POC, Proof-of-Concept formulation (same as group #15 from the dose optimization DOE); DMPC, DPPC, DSPC, DOPC refers to acyl chain structure of liposomal lipids; High, Mid, Low refers to phospholipid: PEGylated lipid ratios. See Supplementary Table 7 for additional formulation composition details. **See Supplementary Table 8 for detailed adjuvant stability results. Color scale: higher desirability index values are light blue, lower desirability index values are dark blue.