Figure 1.

Design of the INSTIGO study. Patients will have blood samples taken at diagnosis, on the day of first-line surgery or post-NAC surgery, on the day of the start of radiotherapy, and 6 months and one year after radiotherapy. If necessary, a blood sample will be taken at the time of diagnosis of metastatic relapse. Blood samples will be used for circulating proteins quantification, blood cells assay and circulating tumour DNA quantification. In neoadjuvant setting, TILs rate will be evaluated from tumour tissue from the biopsy and from the operating room.