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PDAC cells, but not leukocyte subsets, cleave ADAM9 substrates. (A) Schematic overview of ADAM9 substrate containing a 5’ fluorophore (FAM) and a 3’quencher (Dabcyl). (B) ADAM9 substrate cleavage by recombinant ADAM9 and thrombin (negative control). (C) ADAM9 substrate cleavage by CM derived from PDAC cells. (D) Lack of ADAM9 substrate cleavage by leukocyte-derived CM (with PANC1 CM as positive control).
