Skip to main content
. 2021 Jul 3;13(13):3347. doi: 10.3390/cancers13133347

Figure 5.

Figure 5

Inhibition of miR-423-5p mediates the NGF-induced stimulation of MMP-2 expression and the migratory activity of human chondrosarcoma cells. (A,B) MiRNA target prediction software was used to identify miRNAs that potentially bind to the MMP-2 3′-UTR. (C) SW1353 and JJ012 cells were incubated with NGF and miR-423-5p levels were examined by qPCR. (D–F) Cells were transfected with an miR-423-5p mimic then stimulated with NGF. The cell migration and MMP-2 expression levels were examined by Transwell, wound healing and qPCR. (G,H) Cells were pretreated with FAK and c-Src inhibitors or an siRNA and then stimulated with NGF prior to a qPCR analysis of the miR-423-5p levels. (I) The wild-type and mutant MMP-2 3′-UTRs contained the miR-423-5p binding site. (J) Cells were transfected with 3′-UTR plasmids as indicated then stimulated with NGF. The luciferase activity was examined. * p < 0.05 compared with the control group; # p < 0.05 compared with the NGF-treated group.