Figure 5.

Honokiol, a specific SIRT3 inducer, attenuates MLE-12 cell oxidant-induced mtDNA damage in a SIRT3-dependent manner. Forty-eight hours after transfection with scramble or Sirt3 siRNA, MLE cells were treated for 6 h with 10 µM honokiol, followed by 24-h treatment with amosite asbestos @ 25 µg/cm² or 250 µM H₂O₂. mtDNA damage expressed as lesion frequency per DNA fragment. All n = 3; * p = 0.07 vs. scramble + Asb or H₂O₂; † p < 0.05 vs. honokiol + Asb, or honokiol + H₂O₂.