Figure 10.

Metabolic changes of HuH-7 cells after treatment with APAP (5 mmol L⁻¹), CeO₂ NPs (100 µg mL⁻¹) and APAP (5 mmol L⁻¹) plus CeO₂ NPs (100 µg mL⁻¹). The diagram shows the values of the discriminant scores obtained from Fisher’s discriminant analysis of 24 HuH-7 samples for 78 principal compounds, which were selected to discriminate between cells treated with APAP (5 mmol L⁻¹), CeO₂ NPs (100 µg mL⁻¹) and APAP (5 mmol L⁻¹) plus CeO₂ NPs (100 µg mL⁻¹). All four groups can be well separated from each other. The model was evaluated using the “leave-one-out” formalism (100% correct grouping of ungrouped cases).