Figure 6.

Flow diagram of phenotyping protocol. (A) Sterilized wheat seeds for germination; (B) germinated seeds after two days; (C) transferring healthy seedlings to pot containing perlite and vermiculite; (D) transplanted seedlings; (E) different stages of seedlings growth under controlled condition; (F) manual measurement of root and shoot length; (G) root images captured by using flatbed scanner; (H) scanned images of roots. The experimental design adopted for screening was a completely randomized design (CRD) with three replications including two checks (C306 and HD2967). For each replication five biological replicates were used in each pot. The experiment was done in batches for 20 genotypes at each time. Before germination, 20 seeds of each accession were washed carefully and thoroughly with double distilled water and surface sterilized with 0.5% Sodium hypochlorite solution for 30 s. The sterilized seeds were then washed with double distilled water three to four times to remove any trace of adhering chemicals. The seeds were placed well spread in a thoroughly moist germination paper/filter paper taken in a petri dish and allowed to germinate under a growth chamber at 22 ± 1 °C room temperature in the dark.