Skip to main content
. 2021 Jun 24;22(13):6782. doi: 10.3390/ijms22136782

Figure 2.

Figure 2

Effect of Y-27632 on the cytoskeletal dynamics and apoptosis of RLKs. (A) Experimental scheme. Cells were continuously cultured in MTCM with Y-27632 till passage 5. Then, Y-27632 was removed from the culture medium when cells reached 60–70% confluence on day 0 and re-added to the culture medium on day 3. Cells on day 0, 1, 3, and 6 were harvested for analysis. (B) Cytoskeletal reorganization in RLKs following Y-27632 removal and re-addition. Actin filaments were stained with Phalloidin-iFluor 488 (green) and nuclei were stained with DAPI (blue). Scale bars, 50 μm. (C) Representative images of TUNEL-positive nuclei (green) and DAPI-stained nuclei (blue) in RLKs at different time points. DNAse-treated cells were used as a positive control. Scale bar, 100 μm. (D) Quantification of TUNEL-positive cells (n = 6 six random separate fields; n.s. = non-significant; one-way ANOVA). (E) Relative nucleus area of RLKs at different time points. Data were normalized with the average nucleus size on Day 0 (n = 6 six random separate fields; **** p < 0.0001, ** p = 0.0017, n.s. = non-significant; one-way ANOVA).