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. 2021 Jun 25;12:679854. doi: 10.3389/fmicb.2021.679854

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Copyright © 2021 Hwang, Im and Choi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of the mutation in the palindromic sequence on the BrpR-His6 binding. (A) The sequences of 38-bp DNA oligonucleotides which contain the wild-type (BRPBwt) or mutated (BRPBmt1, BRPBmt2, and BRPBmt3) palindromic sequences for the BrpR-His6 binding are shown. The palindromic sequences are shown in bold, and the mutated bases are shown in red. (B) The 6-FAM-labeled DNA oligonucleotides were incubated with increasing amounts of BrpR-His6 as indicated in the presence of c-di-GMP (50 μM). B, bound DNA; F, free DNA.