FIGURE 2.

BrpR activates brpT in a growth phase and c-di-GMP-dependent manner. (A) Total RNAs were isolated from the parent strain grown to an A₆₀₀ of 0.5 (exponential phase) or 2.0 (stationary phase) with or without 0.01% arabinose. The brpR expression was determined by qRT-PCR analysis, and the brpR expression in the exponential phase without arabinose was set at 1. (B) Total RNAs were isolated from the parent and ΔbrpR strains grown to an A₆₀₀ of 0.5 (exponential phase) or 2.0 (stationary phase) with 0.01% arabinose. The brpT expression was determined by qRT-PCR analysis, and the brpT expression of the parent strain in the exponential phase was set at 1. Error bars represent the SD. Statistical significance was determined by the Student’s t test (**, P < 0.005; *, P < 0.05; ns, not significant).