FIGURE 4.

Effects of BrpR and BrpT on the expression of downstream genes. Total RNAs were isolated from the parent and mutant strains grown to an A₆₀₀ of 2.0 with 0.01% arabinose. The brpR, brpT, brpA, brpL, brpG, and VV1_2302 expression was determined by qRT-PCR analysis, and the expression of each gene in the parent strain was set at 1. ND, not detected. Parent, parent strain; ΔbrpR, ΔbrpR mutant; ΔbrpT, ΔbrpT mutant; ΔbrpRΔbrpT, ΔbrpRΔbrpT double mutant. Error bars represent the SD. Statistical significance was determined by the Student’s t test (**, P < 0.005; *, P < 0.05; ns, not significant).