Fig. 6.

CircMTO1 overexpression attenuated arteriosclerosis by sponging miR-182-5p in vivo. A Representative microscopic images of the aortic root of a 36-week-old ApoE^−/− mice injected by AAV-modified vector via tail vein (AAV-PCDNA, AAV-pcDNA-circMTO1 and AAV-pcDNA-circMTO1/AAV-miR-182-5p). Lipid fixation was performed followed by oil red O (red) and smooth muscle α-actin (SMA, yellow) staining. B Western blot results of α-SMA, LDLR (LDL receptor), RASA1 and GADPH in the AS mice (AAV-PCDNA, AAV-pcDNA-circMTO1 and AAV-pcDNA-circMTO1/AAV-miR-182-5p). * vs NC group, ^# vs AS group, ^&vs AS + circMTO1, **P < 0.01, *P < 0.05, ^##P < 0.01, ^#P < 0.05, ^&&P < 0.01, ^&P < 0.05