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. 2021 Jul 1;22(13):7145. doi: 10.3390/ijms22137145

Figure 3.

Figure 3

Inhibition of ANO1 channel activity by resveratrol, curcumin, xanthohumol, and isoxanthohumol. The activity of ANO1 was measured in Fischer rat thyroid (FRT) cells stably co-expressing human ANO1 and the yellow fluorescent protein (YFP). After the cells were exposed to positive controls (NPPB and T16Ainh-A01) or one of the four compounds for 20 min, they were treated with 140 mM NaI solution containing 100 µM ATP to activate ANO1 channel activity, continuously measured every 0.2 s for 6 s. (a) Fluorescence intensity of cells exposed to vehicle, positive controls, resveratrol, and curcumin. (b,d) Relative fluorescence intensity obtained from tested cells at 6 s. (c) Fluorescence intensity of cells exposed to vehicle, positive controls, xanthohumol, and isoxanthohumol. To compare the differences among vehicle, positive controls, resveratrol, curcumin, xanthohumol, and isoxanthohumol, two-way ANOVA and Tukey’s post-hoc tests were used. *: significantly different from vehicle (* p < 0.05, ** p < 0.01, and *** p < 0.001).