Figure 6.

Changes in ANO1 and immune response-related cytokine mRNA levels in mouse ears showing psoriasis-like symptoms caused by inhibiting ANO1. Two mouse groups (IMQ [n = 7] and IMQ + A01 [n = 7]) received a daily dose of 25 mg of 5% IMQ cream on right ears for 7 consecutive days, and the IMQ + A01 group additionally received 15 µL of 100 µM A01 at 30 min before and after IMQ treatment. The control group received a similar dose of Vaseline cream on their right ears. After extracting total RNA from the right ears of the mice (7 animals in each group), obtained on the eighth-day post-treatment onset, target genes were analyzed using RT-PCR.; their expression levels were measured and represented as densitometric graphs. To compare the differences among groups, one-way ANOVA and Tukey’s post-hoc tests were used. *: significantly different between two groups (* p < 0.05, ** p < 0.01, and *** p < 0.001). Imiquimod, IMQ; T16Ainh-A01, A01.