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. 2021 Jun 23;22(13):6760. doi: 10.3390/ijms22136760

Figure 1.

Figure 1

Effects produced by intra-nigral injection of AAVs coding for various forms of the C-terminal fragment of LRRK2. (A) Various forms of the C-terminal fragment of LRRK2 (∆LRRK2) were cloned into an AAV backbone with the PGK promoter: the wild-type form (WT), the pathological form with the G2019S S substitution (GS), or the dead kinase form of G2019 with the D1994A mutation (DK). AAVs were unilaterally injected into the rat SNpc. The cylinder test, which assesses forepaw asymmetry use, was performed at 15 weeks post-injection (PI), and the rats were processed for histological evaluation (ICH). (B) Results of the cylinder test at 15 weeks PI. (C) Representative photomicrographs of sections of the various experimental groups labeled using Tyrosine Hydroxylase (TH) immunohistochemistry. (D) Number of TH-positive neurons in the SNpc measured using unbiased stereology. Results are expressed as the means ± the SEM. N = 8–12 animals/group. ANOVA and PLSD post hoc test. n.s.: not significant. ** p < 0.01. Scale bars: 750 µm left panel and 400 µm right panel in (C).