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. 2021 Jun 23;22(13):6760. doi: 10.3390/ijms22136760

Figure 5.

Figure 5

Immunohistochemistry for tyrosine hydroxylase (TH) and p-synS129-positive (p-synS129) cells and axons at 15 and 6 weeks post-injection. (A–C) Histological images and graphical quantification of TH-positive cells in the SNpc at 15 weeks PI (A,B) and 6 weeks PI (G,H). (E–H) Histological images and graphical quantification of p-synS129-positive (p-synS129+) neurons in the SNpc at 15 weeks PI (C,D) and 6 weeks PI (I,J). The number of TH-positive and p-synS129-positive cells was evaluated using unbiased stereology at very high magnification. (I–L) Histological images and graphical quantification of rat brain sections labeled by p-synS129 immunohistochemistry at the level of the striatum at 15 weeks PI (E,F) and 6 weeks PI (K,L) showing the presence of sparse positive objects with a necklace-like organization. The quantification was determined as the percentage of the field of view (area) occupied by p-synS129-positive staining. Results are expressed as the means ± SEM. N = 7–13 animals/group. ANOVA and PLSD post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bars: 400 in (A,C,E,G) and 50 µm in images in (I,K).