Figure 6.

Tyrosine hydroxylase (TH) levels in the striatum of rats injected with AAV-α-syn^A53T with AAV-GFP or ΔLRRK2^G2019S in the right SNpc. (A) Photomicrographs at low magnification showing immunofluorescence for TH (red) and GFP (green) in the striatum at 15 weeks PI. Scale bar: 1000 µm. (B) Photomicrographs at two different magnifications (5× and 63×) showing TH-related immunofluorescence in the striatum of rats injected with PBS or AAV-α-syn^A53T with AAV-GFP, as a control, or ΔLRRK2^G2019S (GS). Scale bar in B: 5×, 1000 µm, 63×, 100 µm. (C) Quantification of fluorescence in the striatum. Quantification was performed at 5× magnification. Results are expressed as the means ± SEM. N = 10 animals/group. ANOVA and PLSD post hoc test. ** p < 0.01.