Figure 8.

Co-localization and expression of ΔLRRK2 and α-syn^A53T 6 weeks after the co-injection of AAV-α-syn^A53T with either AAV-ΔLRRK2^G2019S or AAV-ΔLRRK2^{G2019S/D1994A}. (A,B) Photomicrographs showing the results of immuno-fluorescence detection of ΔLRRK2^G2019S (ΔLRRK2^GS) or ΔLRRK2^{G2019S/D1994A} (ΔLRRK2^DK–red channel) and α-syn (green channel). Left and right images were obtained at low (A) and high (B) magnification, respectively. Note that most neurons express both transgenes. Scale bars: 200 µm for the top images, 50 µm for the bottom images in (A), and 10 µm for the bottom images in (B). (C) Quantification of the percentage of co-localization (upper histogram), α-syn fluorescence (middle histogram), and ΔLRRK2 fluorescence (bottom histogram) based on the analysis of 20 cells per animal, 180 cells in total. Results are expressed as the means ± SEM. N = 4–5 animals/group. No statistical difference between groups, Student’s t-test.