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. 2021 Jul 8;21:360. doi: 10.1186/s12935-021-02019-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Wnt/β-catenin pathway participates in the maintenance of OCSCs. OCSCs stably transfected with sh1 were delivered miR-30a inhibitor or SOX9-vector, or 5 mM Wnt/β-catenin specific activator SKL2001. A, B. Western blot analysis detected the expression of Wnt/β-catenin pathway; C. Microscopic observation of spheres (100 ×) and sphere-forming rate of OCSCs measured by sphere-forming assay; D Relative levels of CD133, SOX9, CD44, NANOG, and POU5F1 in CD133⁺ OCSCs were measured using Western blot analysis; E Cell apoptosis was measured by flow cytometry; F Relative Caspase-3 activity; G. Relative percentage of DNA damage in cell supernatant of each group. VS the sh1 + PBS group, * p < 0.05. Data in panels A and B were analyzed using one-way ANOVA and Tukey’s multiple comparison test, while data in panels C and F were analyzed using the unpaired t test. The experiment was performed in triplicate and data were expressed as means ± standard deviation