Table 2.
Primer sequence of RT-qPCR
| Gene | Sequence 5′–3′ | |
|---|---|---|
| CD133 | F: AGTCGGAAACTGGCAGATAGC | R: AGTCGGAAACTGGCAGATAGC |
| LINC00115 | F: TGGCTTGTCTTCCATCGTCC | R: GCACGAGGGTTGTTACAGGA |
| CD44 | F: CGGACACCATGGACAAGTTT | R: CCGTCCGAGAGATGCTGTAG |
| GAPDH | F: CAAGATCATCACCAATGCCT | R: CCCATCACGCCACAGTTTCC |
| NANOG | F: TTTGTGGGCCTGAAGAAAACT | R: AGGGCTGTCCTGAATAAGCAG |
| POU5F1 | F: CTGGGTTGATCCTCGGACCT | R: CCATCGGAGTTGCTCTCCA |
| miR-30a | F: GCGACTGTAAACATCCTCGAC | R: CAGCTGCAAACATCCGACTG |
| SOX9 | F: AGGAAGTCGGTGAAGAACGG | R: AGGAAGTCGGTGAAGAACGG |
| SOX9-3’UTR-Primer | F: CGGACTAGTACAGACCTTAATCTTAATTA | R: CCCAAGCTTTTTTTAATGCAATGTATATT |
| SOX9-mut-Primer | F: GACTAGTTATTAACAGTTTTAGAAGTC | R: CAAGCTTAATGCAATGTATATTTATTG |
| Luc-LINC00115wt-Primer | F: CGGACTAGTATCAGCAGAGATATGGAGGA | R: CCCAAGCTTGACAATCCAGGTCATCCTTC |
| Luc-LINC00115mut-Primer | F: GACTAGTGTAGCAGAGAGGGCAGCAGA | R: CAAGCTTTTGATACTTATACTTAGTTG |
| U6 | F: CTCGCTTCGGCAGCACATATACT | R: ACGCTTCACGAATTTGCGTGTC |
RT-qPCR, reverse transcription quantitative polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; miR-30a, microRNA-30a