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. 2021 Jun 25;22(13):6829. doi: 10.3390/ijms22136829

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Curcumin suppresses the TGF-β1-mediated angiogenic activity in EA.hy926 endothelial cells. (A) Viability of EA.hy926 endothelial cells (ECs) was measured after treatment with conditioned medium from curcumin or TGF-β1-treated orbital fibroblasts. The fibroblasts were seeded in 10% FBS-containing medium overnight and then treated with curcumin or TGF-β1 for 24 h. The medium was then discarded and replenished with the basal DMEM for an additional 24 h as the conditioned medium for treatment of ECs. Data are expressed as the mean ± SD (n = 3). ns, not significant. (B) The relative tube-branching numbers of ECs cultured at indicated conditioned medium from orbital fibroblasts. (4× field) Data are expressed as the mean ± SD (n > 3). *, p < 0.05.